Background

STR (Short Tandem Repeat) refers to a special pattern in DNA sequence, that is, a string of short nucleotide sequences are repeated. Since each individual's STR sequence is unique, they can be used as genetic markers for individual identification. STR typing detection technology is widely used in forensic medicine, anthropology, biology and other fields. Its main function is to be used for individual identification, paternity testing, and genetic susceptibility research of diseases.

In terms of individual identification, STR testing is widely used in the field of forensic medicine, such as criminal investigation, identification of missing persons, and identification of disaster remains. At the same time, STR testing is also the gold standard method for paternity testing, and its accuracy and reliability have been widely recognized.

In the study of animal and plant population genetics, STR analysis can be used to study the genetic diversity, inbreeding and genetic drift of animal and plant populations.

At present, the most widely used application of STR is still cell identification. In 2011, the American National Standards Institute issued a special cell STR identification standard, and the ChP (Pharmacopoeia of the People's Republic of China) also stipulates STR tests for cell line quality control. STR genotyping has been used as the gold standard for cell identification by authoritative organizations such as ICLAC and ATCC.

Principle of the method

STR identification uses PCR (polymerase chain reaction) technology to amplify specific STR sites, and then separates amplified fragments of different lengths by electrophoresis. Since different individuals have different numbers of repetitions at specific STR sites, the lengths of the amplified fragments generated are also different. By comparing these lengths, the genetic characteristics of the individual can be determined, and then a certain calculation method can be used to obtain the STR typing results.

Product data

In STR experiments, how to judge the accuracy of the results, standards are essential. Kebai now launches STR-related standards, which mainly include 1. Cell identification: autosomal series and sex chromosome series, as well as species contamination. 2. Reproductive inheritance: chromosome aneuploidy pairing standards (mother and fetus) and maternal cell contamination detection standards.

1. Autosomal series, divided into two standards: male (9948) and female (9947): Contains 20 commonly used STR loci (including 13 CODIS loci) and one gender locus Amelogenin.

STR Reference Standard-1 CBPO0001 

STR Reference Standard-2 CBPO0002

2. Sex chromosome series:

X chromosome standards contain 19 X-STR loci and one sex locus, Amelogenin. Y chromosome standards contain 22 low-frequency mutation Y-STR loci and 7 high-frequency mutation Y-STR loci. The combination of low-frequency mutation and high-frequency mutation loci has higher individual identification ability, providing strong compatibility and screening capabilities.

X-STR Reference Standard CBPO0003

Y-STR Reference Standard CBPO0004